A compendium of ecological knowledge for restoration of freshwater fishes in Australia’s Murray–Darling Basin

Many freshwater fishes are imperilled globally, and there is a need for easily accessible, contemporary ecological knowledge to guide management. This compendium contains knowledge collated from over 600 publications and 27 expert workshops to support the restoration of 9 priority native freshwater fish species, representative of the range of life-history strategies and values in south-eastern Australia’s Murray–Darling Basin. To help prioritise future research investment and restoration actions, ecological knowledge and threats were assessed for each species and life stage. There is considerable new knowledge (80% of publications used were from the past 20 years), but this varied among species and life stages, with most known about adults, then egg, juvenile and larval stages (in that order). The biggest knowledge gaps concerned early life stage requirements, survival, recruitment, growth rates, condition and movements. Key threats include reduced longitudinal and lateral connectivity, altered flows, loss of refugia, reductions in both flowing (lotic) and slackwater riverine habitats, degradation of wetland habitats, alien species interactions and loss of aquatic vegetation. Examples and case studies illustrating the application of this knowledge to underpin effective restoration management are provided. This extensive ecological evidence base for multiple species is presented in a tabular format to assist a range of readers

Indicate separate contributions of long-lived and short-lived greenhouse gases in emission targets

European Union funding: 821205, 821003, 820829. Wellcome Trust: 205212/Z/16/

Indicate separate contributions of long-lived and short-lived greenhouse gases in emission targets

European Union funding: 821205, 821003, 820829. Wellcome Trust: 205212/Z/16/

Convalescent plasma in patients admitted to hospital with COVID-19 (RECOVERY): a randomised controlled, open-label, platform trial

SummaryBackground Azithromycin has been proposed as a treatment for COVID-19 on the basis of its immunomodulatoryactions. We aimed to evaluate the safety and efficacy of azithromycin in patients admitted to hospital with COVID-19.Methods In this randomised, controlled, open-label, adaptive platform trial (Randomised Evaluation of COVID-19Therapy [RECOVERY]), several possible treatments were compared with usual care in patients admitted to hospitalwith COVID-19 in the UK. The trial is underway at 176 hospitals in the UK. Eligible and consenting patients wererandomly allocated to either usual standard of care alone or usual standard of care plus azithromycin 500 mg once perday by mouth or intravenously for 10 days or until discharge (or allocation to one of the other RECOVERY treatmentgroups). Patients were assigned via web-based simple (unstratified) randomisation with allocation concealment andwere twice as likely to be randomly assigned to usual care than to any of the active treatment groups. Participants andlocal study staff were not masked to the allocated treatment, but all others involved in the trial were masked to theoutcome data during the trial. The primary outcome was 28-day all-cause mortality, assessed in the intention-to-treatpopulation. The trial is registered with ISRCTN, 50189673, and ClinicalTrials.gov, NCT04381936.Findings Between April 7 and Nov 27, 2020, of 16 442 patients enrolled in the RECOVERY trial, 9433 (57%) wereeligible and 7763 were included in the assessment of azithromycin. The mean age of these study participants was65·3 years (SD 15·7) and approximately a third were women (2944 [38%] of 7763). 2582 patients were randomlyallocated to receive azithromycin and 5181 patients were randomly allocated to usual care alone. Overall,561 (22%) patients allocated to azithromycin and 1162 (22%) patients allocated to usual care died within 28 days(rate ratio 0·97, 95% CI 0·87–1·07; p=0·50). No significant difference was seen in duration of hospital stay (median10 days [IQR 5 to >28] vs 11 days [5 to >28]) or the proportion of patients discharged from hospital alive within 28 days(rate ratio 1·04, 95% CI 0·98–1·10; p=0·19). Among those not on invasive mechanical ventilation at baseline, nosignificant difference was seen in the proportion meeting the composite endpoint of invasive mechanical ventilationor death (risk ratio 0·95, 95% CI 0·87–1·03; p=0·24).Interpretation In patients admitted to hospital with COVID-19, azithromycin did not improve survival or otherprespecified clinical outcomes. Azithromycin use in patients admitted to hospital with COVID-19 should be restrictedto patients in whom there is a clear antimicrobial indication

Fish Distribution in Far Western Queensland, Australia: The Importance of Habitat, Connectivity and Natural Flows

The endorheic Lake Eyre Basin drains 1.2 million square kilometres of arid central Australia, yet provides habitat for only 30 species of freshwater fish due to the scarcity of water and extreme climate. The majority are hardy riverine species that are adapted to the unpredictable flow regimes, and capable of massive population booms following heavy rainfall and the restoration of connectivity between isolated waterholes. The remainder are endemic specialists from isolated springs with very restricted ranges, and many are listed under relevant state and national endangered species legislation and also by the International Union for Conservation of Nature (IUCN). For these spring communities, which are sustained by water from the Great Artesian Basin, survival is contingent on suitable habitat persisting alongside extractive mining, agriculture and the imposition of alien species. For the riverine species, which frequently undertake long migrations into ephemeral systems, preservation of the natural flow regime is paramount, as this reinstates riverine connectivity. In this study, fish were sampled from the Bulloo River in the east to the Mulligan River in the west, along a temporal timeframe and using a standard set of sampling gears. Fish presence was influenced by factors such as natural catchment divides, sampling time, ephemerality and the occurrence of connection flows and flooding. Despite the comparatively low diversity of species, the aquatic systems of this isolated region remain in good ecological condition, and as such they offer excellent opportunities to investigate the ecology of arid water systems. However, the presence of both endangered species (in the springs) and invasive and translocated species more widely indicates that active protection and management of this unique area is essential to maintain biodiversity and ecosystem integrity

Genetic structure and effective population size of the most northern population of the Australian River Blackfish, Gadopsis marmoratus (Richardson 1848): Implications for long-term population viability

Upland freshwater habitats support populations that are especially susceptible to anthropogenic change. Furthermore, their isolation from other suitable habitats, and the fragmented, dendritic structure of headwaters make dispersal an unlikely response to change. We investigated genetic structure and variation in the northernmost population of Gadopsis marmoratus, which is isolated in a tiny area in the headwaters of the Condamine River catchment, in the Murray - Darling Basin, Australia. Strong genetic structure was detected among subpopulations based on microsatellites (FST 5 0.173, p \u3c 0.0001) and mitochondrial (mt)DNA (FST 5 0.369, p \u3c 0.05). Effective population size was low, ranging between 18.8 and 48.2, depending on the estimation method used. Bayesian clustering revealed 3 genetic clusters, but they were not congruent with drainage patterns, suggesting a complex history of dispersal among headwaters that are isolated by waterfalls. Overall, these results suggest that G. marmoratus is unlikely to disperse into new habitats if local conditions become unsuitable. Low effective population size and genetic diversity also suggest that local adaptation would be unlikely

Multiscale relationships between stream temperature and juvenile recruitment in an imperilled freshwater fish

The distribution and population structure of organisms is governed by a broad suite of biotic and abiotic variables, interacting across multiple scales. Recruitment is a key demographic process critical to the maintenance of successful populations. Isolating and quantifying the multiscale environmental drivers of recruitment is vital for species conservation, especially for those species with traits that increase their susceptibility to local extirpation. We developed a Bayesian hierarchical model to quantify the relationship between the rate of recruitment in a locally threatened river blackfish (Gadopsis marmoratus) and environmental predictors across two spatial scales. There was a 0.99 probability that increased broad-scale stream temperature negatively affected juvenile recruitment rate. We also found that there was a 0.97 probability that the fine-scale relationship between recruitment rate and riparian foliage cover was dependent on temperature. This suggests that broad-scale thermal conditions provide the template upon which at least one local environmental variable influences recruitment rate. Understanding drivers influencing key population processes and the spatial scales at which they operate is critical to gaining an insight into likely changes in population persistence for potentially imperilled species, along with the potential future effects of habitat degradation and climate warming on freshwater fishes in general. </p